Recombinant DNA Technology and Gene Cloning

What this note covers

1. What is Recombinant DNA Technology?
2. Step 1 — Restriction Enzymes: Cutting the DNA
3. Step 2 — Vectors: The Plasmid Carrier
4. Step 3 — Ligation: Joining the DNA
5. Step 4 — Transformation: Inserting the Recombinant Plasmid into the Host
6. Step 5 — Selection, Screening, and Expression
7. Overview Summary and Applied NSW HSC Example

7 sections · 14 key terms & formulas · 6 common mistakes