QCAA Biology 2025 General Senior Syllabus (applies from the 2026 cohort; the 2019 syllabus was examined 2020-2025)
Biology is assessed through internal assessments completed at school across Units 3 and 4, plus a QCAA external assessment that draws only on Unit 3 and Unit 4 subject matter. The external assessment is split across two papers. Paper 1 is issued as a multiple choice question book together with a separate question and response book, and Paper 2 is a question and response book of longer written items. QCAA publishes a marking guide and a subject report alongside each year's papers.
Past papers on this subject span more than one syllabus. Papers written under an older one still work as practice, but the units and topics they test have changed — the index labels every paper with the syllabus it was set under.
Biology 2019 syllabus (v1.2 → v1.3) · 2020–2025Biology 2025 syllabus (v1.3) · 2026–present
The units and topics, one by one
Each area below lists the concepts named in the syllabus, what the QCAA exam asks of them, and the mistake that most often costs marks.
Area 1 of 4
Unit 3 Topic 1: Biodiversity and populations
This topic is about putting a number on biodiversity rather than describing it. You work with species richness, the count of different species present, and species evenness, the relative abundance of each, then quantify vegetation using percentage cover and percentage frequency before combining richness and evenness into Simpson's diversity index. Running alongside is the question of why species live where they do: the abiotic and biotic factors that limit distribution and abundance, from temperature, salinity, light and water availability through to competition and predation, and the way tolerance limits define a zone of optimal survival. The topic closes on biological classification, working through the Linnaean hierarchy from domain down to species and weighing molecular evidence such as DNA and protein sequence comparison against morphological evidence when organisms are grouped or reclassified.
What the syllabus lists under this area · 3 points
- Measuring biodiversity (species richness, evenness, percentage cover/frequency, Simpson's diversity index)
- Environmental factors limiting distribution and abundance of species
- Biological classification and taxonomy (Linnaean hierarchy, molecular and morphological evidence)
What the exam asks
Expect field data you have not seen before: a quadrat table to convert into richness, evenness or a Simpson's index value, or a distribution graph to explain against a named limiting factor. Multiple choice items test classification rules and index interpretation, while longer items ask you to justify a taxonomic placement or explain abundance changes along an environmental gradient.
Where marks go missing
Calculating Simpson's index correctly and then never interpreting it, since a bare number earns little. Students also treat richness and evenness as the same thing, calling two communities equally diverse when their abundance distributions differ sharply.
Area 2 of 4
Unit 3 Topic 2: Functioning ecosystems and succession
Here an ecosystem is treated as a working system of energy and matter. You learn to classify one using stratified sampling and field data, then trace energy as it is transformed and lost through trophic levels, which is where biomass and energy pyramids and transfer efficiency come in. Matter is traced separately: the water, carbon and nitrogen cycles, including the microbial steps of nitrogen fixation, nitrification and denitrification. The topic then narrows to the roles organisms play, distinguishing habitat from ecological niche, identifying keystone species, and classifying interactions such as predation, competition, mutualism and parasitism. Population dynamics adds the quantitative layer with growth rate, carrying capacity, exponential and logistic growth, and mark-recapture estimates using the Lincoln index. Finally, ecological succession explains how communities replace one another over time, contrasting primary succession on bare substrate with secondary succession after disturbance.
What the syllabus lists under this area · 6 points
- Stratified sampling and field data collection to classify an ecosystem
- Energy flow and transformation through trophic levels (biomass, energy pyramids)
- Matter cycling through ecosystems (water, carbon, nitrogen cycles)
- Ecological niche, keystone species and species interactions
- Population dynamics — carrying capacity, growth rate, Lincoln Index (mark–recapture)
- Ecological succession (primary and secondary)
What the exam asks
Calculation and interpretation dominate: a Lincoln index estimate from capture data, an energy transfer efficiency between trophic levels, or a growth curve to read for carrying capacity. Written items ask you to explain a nutrient cycle step, predict the consequence of removing a keystone species, or account for the sequence of species in a succession.
Where marks go missing
Using the Lincoln index without acknowledging its assumptions, such as no births, deaths or migration between samples and no trap-shyness. Responses that ignore these assumptions cannot answer the evaluation part of the question.
Area 3 of 4
Unit 4 Topic 1: DNA, genes and the continuity of life
This is the molecular and inheritance core of Unit 4. It starts with DNA structure and semi-conservative replication, including the specific jobs of DNA helicase, DNA polymerase and ligase, then moves to gene expression through transcription and translation, reading codon tables and tracking the roles of mRNA, tRNA and ribosomes. Mutation is handled causally: how substitutions, insertions and deletions alter codons, amino acid sequence, protein folding and therefore function. Meiosis is studied for the mechanisms that generate variation, particularly crossing over and independent assortment. Mendelian genetics then covers monohybrid and dihybrid crosses, Punnett squares, test crosses, codominance, incomplete dominance and sex linkage, along with pedigree interpretation. The topic finishes with biotechnology, covering recombinant DNA and restriction enzymes, the polymerase chain reaction, gel electrophoresis, and the use of DNA profiling and sequencing in identification and diagnosis.
What the syllabus lists under this area · 6 points
- DNA structure, replication and the roles of DNA helicase/polymerase
- Gene expression — transcription and translation
- Mutation and its effects on protein structure/function
- Meiosis, crossing over and genetic variation
- Mendelian inheritance — monohybrid/dihybrid crosses, Punnett squares, codominance, incomplete dominance, sex linkage
- Biotechnology — recombinant DNA, PCR, gel electrophoresis, DNA profiling and sequencing
What the exam asks
Genetics is worked rather than recalled. You may transcribe and translate a given DNA sequence, deduce genotypes from a pedigree, complete a dihybrid cross and state ratios, or interpret a gel electrophoresis banding pattern. Explanation items typically ask how a stated mutation changes a protein, or why a biotechnology technique produces the result shown in the stimulus.
Where marks go missing
Answering mutation questions by jumping straight from a base change to an organism-level effect. Marks sit in the chain: altered base, altered codon, altered amino acid, altered folding and active site, altered function. Missing links lose the explanation marks.
Area 4 of 4
Unit 4 Topic 2: Continuity of life on Earth
This topic scales genetics up to populations and deep time. It begins with the evidence base for evolution, including comparative anatomy, biogeography, developmental biology and molecular data, and with natural selection as a mechanism acting on existing variation. Microevolution covers the four forces that change allele frequencies, mutation, gene flow, genetic drift and selection, along with bottleneck and founder effects and the use of the Hardy-Weinberg principle to test whether a population is evolving. Macroevolution looks at broader patterns of diversification, distinguishing divergent, convergent and parallel evolution and coevolution. Speciation is treated mechanistically through allopatric, sympatric and parapatric modes and the geographic, reproductive and temporal isolating mechanisms that keep gene pools separate. The topic closes with evidence for the origin and history of life on Earth, including phylogenetic trees, the fossil record and molecular clock dating.
What the syllabus lists under this area · 5 points
- Evidence for evolution and the theory of evolution by natural selection
- Microevolution — mutation, gene flow, genetic drift, Hardy-Weinberg principle
- Macroevolution and patterns of diversification (divergent, convergent, parallel evolution, coevolution)
- Speciation — modes (allopatric, sympatric, parapatric) and isolating mechanisms (geographic, reproductive, temporal)
- Evidence for the origin and history of life on Earth (phylogenetics, fossil record, molecular clocks)
What the exam asks
Common tasks include Hardy-Weinberg calculations of allele and genotype frequencies, interpreting or constructing a phylogenetic tree from sequence data, and explaining a described scenario in terms of a named speciation mode. Longer responses ask you to argue which evolutionary mechanism best accounts for the pattern in the stimulus, and to justify that choice against alternatives.
Where marks go missing
Teleological wording, such as saying a population developed a trait because it needed one or that individuals adapted to the change. Variation must already exist and selection acts on it. Purpose-driven phrasing costs marks even when the underlying idea is understood.