PCR, gel electrophoresis, DNA profiling and gene technologies (CRISPR, transgenic organisms)

What this note covers

1. Extraction and amplification solve different problems
2. PCR uses a repeated temperature-controlled cycle
3. Electrophoresis separates fragments for comparison
4. Profiles and sequencing traces answer different questions
5. Gene transfer requires selection, joining and delivery
6. CRISPR targeting and responsible interpretation
7. Interpreting controls in a PCR and electrophoresis investigation
8. Following an intended gene edit from DNA change to evidence

8 sections · 10 key terms & formulas · 6 common mistakes