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Define meiosis
A reductional cell division producing four haploid gametes from one diploid parent cell. Two stages: Meiosis I (reductional) and Meiosis II (equational).
What occurs during Prophase I?
Homologous chromosomes pair up (synapsis) to form bivalents. Crossing over occurs between non-sister chromatids. Nuclear envelope breaks down.
Explain meiosis outcomes
Four genetically different haploid gametes produced from one diploid cell. Each has half the chromosome number. Non-identical due to crossing over and independent assortment.
State Mendel's Law of Segregation
Alleles of a gene segregate during gamete formation. Each gamete receives only one allele. During fertilisation, alleles recombine, restoring diploid state.
State Mendel's Law of Independent Assortment
Alleles of different genes assort independently during gamete formation. Segregation of one gene does not influence another.
What is a monohybrid cross?
Genetic cross involving one gene with two alleles. Example: Aa x Aa produces 3:1 phenotypic ratio in F2.
What is a dihybrid cross?
Genetic cross involving two genes with two alleles each. Example: AaBb x AaBb produces 9:3:3:1 ratio if genes assort independently.
Define sex-linked inheritance
Genes on sex chromosomes (X or Y) with different inheritance patterns. Males (XY) hemizygous; females (XX) homozygous or heterozygous.
What is codominance?
Both alleles fully expressed in heterozygote, showing both traits. Example: ABO blood type AB shows A and B antigens.
List ABO blood types and genotypes
Type A: I^A I^A or I^A i. Type B: I^B I^B or I^B i. Type AB: I^A I^B. Type O: ii.
What symbols are used in pedigrees?
Square = male; Circle = female; Filled = affected; Half-filled = carrier. Horizontal line = couples; vertical line = offspring. Generations use Roman numerals.
Interpret autosomal recessive in pedigrees
Affected individuals usually have carrier parents. Both parents carry recessive allele. Trait can skip generations. Both sexes equally affected.
Interpret X-linked recessive in pedigrees
More affected males than females. Passes from carrier mothers to affected sons. Males cannot pass trait to sons. Affected females have affected fathers.
Name DNA replication enzymes
DNA helicase (unwinds), primase (makes RNA primers), DNA polymerase III (adds nucleotides), DNA ligase (seals Okazaki fragments).
Describe semi-conservative replication
New DNA has one original strand and one new strand. Original strands separate and each templates a complementary new strand.
What is transcription?
mRNA synthesis from DNA template by RNA polymerase. Occurs in nucleus. mRNA carries genetic info to ribosomes.
Three stages of transcription
Initiation: RNA polymerase binds promoter. Elongation: adds RNA nucleotides. Termination: stops at terminator; mRNA released.
What is translation?
Protein synthesis from mRNA at ribosome. tRNA transports amino acids. Codons match to tRNA anticodons.
Three stages of translation
Initiation: ribosome assembles at AUG start codon. Elongation: tRNA brings amino acids; peptide bonds form. Termination: stop codon signals end.
What is the genetic code?
Triplet code: three nucleotides (codon) code for one amino acid. 64 codons: 61 for amino acids, 3 stops. Degenerate code.